The MACSQuant Analyzer 10 is a powerful benchtop flow cytometer with 3 lasers and 10 parameters. Common assays include: immunophenotyping, cell signaling, cell health and toxicity screening. Using Flow Cytometry to Assess Protein Aggregation in Bacterial Overexpression Systems Application Note. The method relies on stacking biomarkers that are not expressed on the same cell and a gating technique to interrogate the populations of interest. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Also includes step-by-step instructions for setting up the CytoFLEX for multicolor immunophenotyping including use of the Compensation Library. Topics important to the validation of a counting assay are discussed. Attributes of each dye are evaluated to aid in selection during panel design. Calcium Flux Assay by Flow Cytometry Application Note. Experimental considerations for nanoscale flow cytometry and illustrated with data from the instrument. The Quanteon is a 4 laser flow cytometer that can be configured with up to 25 independent photomultipliers for meeting the most demanding sample panels. Privacy Statement Merck today launched its new CellStream™ benchtop flow cytometry system, a compact, customizable flow cytometer that uses a camera for detection. Describes a 10 color, 13 marker panel to investigate the NK cell population, both phenotypically and functionally in human PBMC samples. NOT ALL PRODUCTS ARE AVAILABLE IN ALL COUNTRIES. Instructions for reducing background particles found in buffers and reagents to enhance small particle detection. These products are labeled "For Laboratory Use Only." Common assays include: immunophenotyping, … Natural autofluorescence of marine organisms can be detected in flow cytometers. The panel includes the use of an exclusion markers on a dump channel used to gate out non-NK cells. All instruments in the platform utilize CytExpert for CytoFLEX Acquisition and Analysis Software. {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}|{611E05FD-FC41-4E23-9498-DA5662F8D4F9}|{279C6CA2-9797-4CFE-9CAA-5EAFAC29B60D}, Cell Counters, Sizers and Media Analyzers, HIAC PODS+ Portable Industrial Particle Counter, HIAC 9703+ Pharmaceutical Particle Counter, ANATEL A1000 TOC Analyzer for Ultrapure Water, Search Conjugated Antibodies, Antibody Cocktails, Lysing, Fixative, and Permeabilizating Reagents, Plate Loader Options for the CytoFLEX Platform, The ups and downs (and ups) of a powerful research tool: the history of analytical ultracentrifugation (AUC), Customer Relations for Beckman Coulter Life Sciences, CytExpert for CytoFLEX Acquisition and Analysis Software, Unique collection of innovative technologies, Optional 96-well plate sample loader module, Eight Color Immunophenotyping Unlysed Whole Blood Application Note. The traditional barriers of cost, extensive assay development and challenging software have been eliminated, allowing flow cytometry to be integrated directly into the research setting and into protein expression workflows. The light produced by lasers in a flow cytometer is scattered by cells in the sample, measured by detectors, and then translated to signals that can be analyzed and measured. Its unique optics system and design provide researchers with unparalleled sensitivity and flexibility when analyzing cells and submicron particles. Measuring Proliferation in Leukemia Cell Lines Using CFSE Application Note. These systems accommodate smaller sample volumes, generate less waste, have lower operating costs, and are easier to set up and run than traditional flow cytometers. ViaKrome 808 Fixable Viability dye excitation and emission spectrum, with 885/40 bandpass indicated. Bench top flow cytometers Bench top cytometers are fully enclosed. RUO: Research Use Only. Single-cell sorting is completed in 96- or 384-well plates when using the WOLF with the N1 Single Cell Dispenser. A special population of natural killer cells (uNK) has been implicated in preeclampsia. It is designed for hands-free operation with a range of completely automated assays including washing and … ASR: Analyte Specific Reagents. Authors: James Tung, Dan Condello, Albert Donnenberg, Erika Duggan, Jesus Lemus , John Nolan, Kathy Ragheb, Jennifer Sturgis, J Paul Robinson, Zhao, Jing, Domenic Fenoglio, Yijun, Huang, Paul Scibelli | Beckman Coulter, Miami FL, Purdue University Cytometry Laboratories, La Jolla Bioengineering Institute, University of Pittsburgh Cancer Center, Sun Yat-Sen University, School of Medicine, Guangzhou, China, CytoFLEX Instrument Evaluation Using Biological Specimens, Authors: James Tung, Dan Condello, Albert Donnenberg, Erika Duggan, Jesus Lemus, John Nolan, Kathy Ragheb, Jennifer Sturgis, Paul Scibelli, J Paul Robinson, Domenic Fenoglio | Beckman Coulter, Miami FL, Purdue University Cytometry Laboratories, La Jolla Bioengineering Institute, University of Pittsburgh Cancer Center, Assessing T Cell Subpopulations by TCR Expression in PBMCs Application Note. How to use VSSC on CytoFLEX Technical Note. Complete Solutions for your Specific Exosome Workflow from Preparation to Analysis. Benchtop flow cytometry from Luminex offers rapid access to insightful cellular analysis that you can apply to diverse research areas, including immunology and molecular biology. This budget-friendly instrument is capable of detecting up to 17 parameters with enhanced sensitivity and resolution. Along with changes in instrumentation, the commercialization of turnkey assay kits and specialized software has made flow cytometry analysis easy for scientists with no prior experience with the technique. A bead mixture is defined to assist in demonstrating the detection capabilities. Real-world training programs to maximize the value of your flow cytometer investment. Flow cytometry is a technique used to analyze cells for a variety of purposes, including cell counting, phenotyping, cell cycle assessment, and viability. Competitive Mouse Bone Marrow Transplantation Reconstitution Assay Application Note. Provides a detailed protocol for preparing nuclei from plant tissues. Guava Technologies, part of Millipore, developed the first commercially available micro-capillary flow cytometer and helped to launch benchtop flow cytometry. Describes the instrument set up and verification steps required for measuring extracellular vesicles by flow cytometry. Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice Application Note. No Regulatory Status: Non-Medical Device or non-regulated articles. Flow cytometer instruments, antibodies, and reagents from Bio-Rad give you the confidence to do more. The unique peristaltic sample delivery system of the CytoFLEX analyzer enables optimized measurements of transient changes in intracellular calcium in cells following agonist activation. The purpose of this paper is to demonstrate how to setup the CytoFLEX flow cytometer to detect small particles by Violet Side Scatter (VSSC). Sophisticated cell analysis enabled by flow cytometry is now a powerful “on demand” tool for researchers looking for answers to increasingly complex questions about protein activity, function and interaction. Overview. Values obtained are then correlated with the Kew Database to assess ploidy. Despite the power of flow cytometry, the size, complexity and cost of conventional instruments have historically confined this technology to the core lab and limited its use to experts. The technique of flow cytometry, in which cells are optically counted and examined while suspended in a stream of fluid, has emerged as a powerful cell analysis tool that can satisfy requirements for speed and reliability while also providing multiparametric information on individual cells. Solution: Benchtop, micro-capillary flow cytometers, paired with readyto- use cell analysis kits, enable any researcher to leverage the power of flow cytometry, regardless of expertise or access to a core facility. Includes a detailed gating strategy for deep immunophenotyping of T cell populations including rare cell types. easyCyte Flow Cytometers use novel microcapillary technology that enables a compact user-friendly format, which is powerful enough to run complex assays. It boasts on-board fluidics, a sensitivity comparable to other instruments in the lower-end market, and can sort 30,000 cells per second, comparable even to some higher-end sorters.Sony Biotechnology’s SH800 Cell Sorter has at its heart a plastic, disposable microfluidics chip ($25 to $40). document.write(new Date().getFullYear()) Beckman Coulter, Inc. All rights reserved. BD, Accuri, Beckman and more. Nano- and Microparticle Bead Mix for Use as a Sizing Standard in Flow Cytometry. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Provides a detailed procedure for using the Cyto-ID Autophagy kit in a flow cytometry assay. Phenotypic Characterization of Human Natural Killer Cell Populations in Peripheral Blood Application Note. This application note provides a procedure for assessing the cytokine profile using flow cytometry and demonstrates T Helper cell subtypes Th1, Th2, Th17 and a non-classic Th1 type. Assay-specific software modules enable researchers to get meaningful data even more quickly. Maintenance service plan for Beckman flow analyzers. Beckman Coulter provides solutions customized for each step in your specific exosome research workflow – from sample preparation to sample analysis – and can also help improve processes and assist with related challenges. Cellular uptake can be assessed quickly in a large number of cells using flow cytometry. These products are labeled "For In Vitro Diagnostic Use." Because all necessary reagents are included in the kits, no assay development is required. Not for use in diagnostic or therapeutic procedures. The WOLF benchtop flow cytometry cell sorter allows for the gentle separation of cell types in bulk sorting and the isolation and dispensing of individual cells into plates. The CytoFLEX LX Flow Cytometer is an expansion of the CytoFLEX Platform. The first place to look for new and used Flow Cytometers. Sitemap. Do not sell my personal data This document highlights the privacy and security features in the BeckmanConnect remote management solution. Flow Cytometric Quantification to Assess Doral Endocytosis Application Note. Particle scanning and imaging; Single cells up to large colonies; No pre-filtration or pre-processing needed; In situ automated monitoring Identifying Discrete Mouse Splenocyte and Peripheral Blood Cell Subpopulations Using Surface Markers Application Note. Options and upgrades available on the different systems are tailored to address generic, exploratory discovery cell analysis experiments as well as extremely customized separations and cell or particle sorting. MACSQuant VYB Convenient fluorescent protein flow cytometry through an additional optical layout configured with a yellow laser. Please try our Technical Documents search. Paramount for any lab is consistent accurate data. Resolving Dyes with Identical Emission Wavelengths on the CytoFLEX. This assay can be used to assess bacterial culture conditions that favor production of soluble protein. Identifying Brain Tumor Initiating Cells Using Flow Cytometry Application Note. The Guava easyCyte 5 Flow Cytometer offers absolute cell counts and five parameters (3 fluorescent colors, 2 light scatter), from an industry standard blue laser. Fluorescence decreases over time as the dye is divided between daughter cells at each division. These reagents are labeled "Analyte Specific Reagents. CytExpert for CytoFLEX Gain Independent Compensation Flyer, Gain Independent Compensation, a feature of CytExpert acquisition software, is enabled by Avalanche Photodiode detectors and facilitates simplified workflows for immunophenotyping, Protection Plan for your Workflows in Flow Cytometry. In this application note a detailed method for preparing resident NK cells from tissue and an assay to access NK cell activation is provided. More than 20 FlowCellect™ assay kits are available for measuring chemokine receptor levels, stem cell phenotypes, cell viability, apoptosis, cell cycle progression, proliferation and toxicity. Flow Cytometry We are committed to accelerating your science by providing a comprehensive suite of solutions for the analysis of cells and their function by delivering you our flagship flow cytometry products designed to deliver high-performance results and save you … When selecting a flow system, the three common components found in all systems are: fluidics, optics/detecti… MACSQuant Analyzer 16 Automated flow cytometer with expanded fluorescence capabilities, enabling measurement of up to 16 parameters. The proprietary photomultiplier technology employed in the Quanteon provides excellent sensitivity, stability, and a …